Journal: International Journal of Molecular Sciences
Article Title: Thyrotropin-Releasing Hormone Gene Methylation as a Potential Biomarker for Anal Intraepithelial Neoplasia
doi: 10.3390/ijms262411784
Figure Lengend Snippet: TRH methylation analysis. ( A ) Data obtained from GSE186859 dataset, methylation levels at cg01009664 of TRH gene in anal cells with different anal lesions stratified by histology as normal (9 samples), AIN3 (13 samples) and anal cancer (121 samples). ( B ) Pyrosequencing analysis data of normal and cervical cancer cell lines.
Article Snippet: The DNA extracted from human cervical cancer cell lines containing HPV16, i.e., CaSki (ATCC CRL-1550, Manassas, VA, USA) and SiHa (ATCC HTB-35, Manassas, VA, USA), human cervical cancer cell line without HPV; C33A (ATCC HTB-31, Manassas, VA, USA), and normal human cervical epithelial cells, PCS480011 (ATCC PCS-480-011TM, Manassas, VA, USA) were used as a control for hypermethylated and hypomethylated TRH gene.
Techniques: Methylation